miniGC App Note – Phenols (EPA 604)

miniGC App Note – Phenols (EPA 604)

Analysis of Phenols (US EPA Method 604) in the miniGC

Introduction

Phenols or phenolic compounds are toxic compounds that are found in bodies of water that originate from industrial pollution as well as natural decomposition of animal and plant materials.

Reference Method

(https://www.restek.com/en/pages/chromatogram-view/GC_CF01131)

ColumnMXT-5, 30 m, 0.28 mm ID, 0.25 µm
Sample604 Phenols Calibration Mix (Restek PN: 31029)
Conc.25 ng/uL per compound
Injection
Inj. Vol.:1.0 uL splitless injection
Liner:
Inj. Temp.:280°C
Oven
Oven Temp.:Ramp from 40°C to 250°C at 10°C/min
Carrier GasH2, constant pressure
Linear Velocity:50 cm/sec @ 40°C
DetectorFID @ 300°C
Instrument

Reference Standard

604 Phenols Calibration Mix (Restek PN: 31029)

  1. Phenol
  2. 2-Chlorophenol
  3. 2-Nitrophenol
  4. 2,4-Dimethylphenol
  5. 2,4-Dichlorophenol
  6. 4-Chloro-3-methylphenol
  7. 2,4,6-Trichlorophenol
  8. 2,4-Dinitrophenol
  9. 4-Nitrophenol
  10. 2-Methyl-4,6-dinitrophenol
  11. Pentachlorophenol

2,000 ug/mL of each compound in methanol

Reference Chromatogram

Peaks:

  1. Phenol
  2. 2-Chlorophenol
  3. 2-Nitrophenol
  4. 2,4-Dimethylphenol
  5. 2,4-Dichlorophenol
  6. 4-Chloro-3-methylphenol
  7. 2,4,6-Trichlorophenol
  8. 2,4-Dinitrophenol
  9. 4-Nitrophenol
  10. 2-Methyl-4,6-dinitrophenol
  11. Pentachlorophenol

miniGC Method

ColumnMXT-5, 30 m, 0.25 mm ID, 0.25 µm (Restek PN: 70223-LUC25)
Sample604 Phenols Calibration Mix (Restek PN: 31029)
Injection
Inj. Vol.:1.0 µL split injection (split ratio 40:1)
Liner:4 mm low pressure drop precision inlet liner w/ wool (Restek PN: 23309)
Inj. Temp.:250°C
Oven
Oven Temp.:Hold at 50°C for 4 min
Ramp to 250°C @ 5°C/min
Carrier GasHe, constant velocity
Linear Velocity:39.9 cm/sec, 1.90 mL/min
DetectorFID @ 250°C
InstrumentLucidity miniGC

miniGC Chromatograms

Figure 1: Injection 1

Results

This was a very quick experiment to see how closely we could replicate the chromatogram produced on a traditional GC by injecting a standard into the miniGC and comparing the chromatogram to the same chromatogram produced on the traditional GC using the same standard and roughly the same method and column. We used a slightly different method and column for convenience sake, but overall the chromatograms looked very similar for the two systems compared, which is a success. Further experiments are planned.

More results from the miniGC can be found here: https://luciditysystems.com/products/minigc/minigc-results/

More information on the miniGC can be found here: https://luciditysystems.com/products/minigc/

To see the miniGC in operation go here: https://luciditysystems.com/products/minigc/minigc-interface/

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