GC-FID Method Note – Terpenes

Analysis of Terpenes in the miniGC

Introduction

Shown here are a couple of different terpenes mixes run with a few different methods.

miniGC Method 1

Column	MXT-5, 30 m, 0.25 mm ID, 0.25 µm (cat.# 70223-LUC25)
Sample

Injection
Inj. Vol.:	1.0 µL split (split ratio 20:1)
Liner:	4 mm low pressure drop precision inlet liner w/ wool (cat.# 23309)
Inj. Temp.:	250°C
Oven
Oven Temp.:	Stage 1: Hold at 35°C for 0 min Stage 2: Ramp to 205°C at 10°C/min and hold for 0 min Stage 3: Ramp to 360°C at 35°C/min and hold for 2.0 min
Carrier Gas	He, constant pressure
Linear Velocity:	39.0 cm/sec (1.90mL/min)

Detector	FID @ 325°C
Instrument	Lucidity miniGC

Reference Chromatogram

miniGC Method 2

Column	MXT-5, 30 m, 0.25 mm ID, 0.25 µm (cat.# 70223-LUC25)
Sample

Injection
Inj. Vol.:	1.0 µL split (split ratio 10:1)
Liner:	4 mm low pressure drop precision inlet liner w/ wool (cat.# 23309)
Inj. Temp.:	250°C
Oven
Oven Temp.:	Stage 1: Hold at 50°C for 1.0 min Stage 2: Ramp to 300°C at 25°C/min and hold for 4.0 min
Carrier Gas	He, constant velocity
Linear Velocity:	55.6 cm/sec (3.00 mL/min)

Detector	FID @ 300°C
Instrument	Lucidity miniGC

miniGC Chromatograms

miniGC Method 3 – Hydrogen as Carrier Gas

Column	MXT-5, 30 m, 0.25 mm ID, 0.25 µm (cat.# 70223-LUC25)
Sample

Injection
Inj. Vol.:	2.0 µL split (split ratio 20:1)
Liner:	4 mm low pressure drop precision inlet liner w/ wool (cat.# 23309)
Inj. Temp.:	210°C
Oven
Oven Temp.:	Stage 1: Hold at 40°C for 1.0 min Stage 2: Ramp to 230°C at 6°C/min and hold for 4.0 min
Carrier Gas	H₂, constant velocity
Linear Velocity:	47.0 cm/sec (1.80 mL/min)