miniGC App Note – Terpenes

miniGC App Note – Terpenes

Analysis of Terpenes in the miniGC

Introduction

Shown here are a couple of different terpenes mixes run with a few different methods.

miniGC Method 1

ColumnMXT-5, 30 m, 0.25 mm ID, 0.25 µm (cat.# 70223-LUC25)
Sample
Injection
Inj. Vol.:1.0 µL split (split ratio 20:1)
Liner:4 mm low pressure drop precision inlet liner w/ wool (cat.# 23309)
Inj. Temp.:250°C
Oven
Oven Temp.:Stage 1: Hold at 35°C for 0 min
Stage 2: Ramp to 205°C at 10°C/min and hold for 0 min
Stage 3: Ramp to 360°C at 35°C/min and hold for 2.0 min
Carrier GasHe, constant pressure
Linear Velocity:39.0 cm/sec (1.90mL/min)
DetectorFID @ 325°C
InstrumentLucidity miniGC

Reference Chromatogram

Figure 1: Method 1, Injection 1
Figure 2: Method 1, Injection 2

miniGC Method 2

ColumnMXT-5, 30 m, 0.25 mm ID, 0.25 µm (cat.# 70223-LUC25)
Sample
Injection
Inj. Vol.:1.0 µL split (split ratio 10:1)
Liner:4 mm low pressure drop precision inlet liner w/ wool (cat.# 23309)
Inj. Temp.:250°C
Oven
Oven Temp.:Stage 1: Hold at 50°C for 1.0 min
Stage 2: Ramp to 300°C at 25°C/min and hold for 4.0 min
Carrier GasHe, constant velocity
Linear Velocity:55.6 cm/sec (3.00 mL/min)
DetectorFID @ 300°C
InstrumentLucidity miniGC

miniGC Chromatograms

Figure 3: Method 2, Injection 1
Figure 4: Method 2, Injection 1
Figure 5: Method 2, Injection 1

miniGC Method 3 – Hydrogen as Carrier Gas

ColumnMXT-5, 30 m, 0.25 mm ID, 0.25 µm (cat.# 70223-LUC25)
Sample
Injection
Inj. Vol.:2.0 µL split (split ratio 20:1)
Liner:4 mm low pressure drop precision inlet liner w/ wool (cat.# 23309)
Inj. Temp.:210°C
Oven
Oven Temp.:Stage 1: Hold at 40°C for 1.0 min
Stage 2: Ramp to 230°C at 6°C/min and hold for 4.0 min
Carrier GasH2, constant velocity
Linear Velocity:47.0 cm/sec (1.80 mL/min)
DetectorFID @ 300°C
InstrumentLucidity miniGC

miniGC Chromatograms

Figure 6: Method 3, Injections 1-3 overlaid
Figure 7: Method 3, Injection 1
Figure 8: Method 3, Injection 2
Method 3, Injection 3
Figure 10: Method 3 details
Figure 11: Method 3 details
Figure 12: Method 3 details
Figure 13: Method 3 details

More results from the miniGC can be found here: https://luciditysystems.com/products/minigc/minigc-results/

More information on the miniGC can be found here: https://luciditysystems.com/products/minigc/

To see the miniGC in operation go here: https://luciditysystems.com/products/minigc/minigc-interface/

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